Dear Modellers,
I try to model my target protein on several templates (a big one and 6
very small ones), moreless as it is indicated in question 4 of the FACs.
Unfortunately the program ends in error when checking the alignements,
with the following error message:
###################
fit2xyz_E> number of equivalent positions < 3: 0
recover__> MODELLER_STATUS >= STOP_ON_ERROR: 1 1
###################
My alignement file looks like this:
>P1;temp1
structure:temp1: 45:: 356::
NECLGTIGPVTPLDASDFALDIRMPGVT-PKESDTYFCMSMRLPVD-EEAFVIDFKP--------RASMDTVHHMLL
FGC----------------------------N--MPSSTGSYWFCDEGTCTDKAN----ILYAWARNAPPTRLPKGV
GFRVGGETGSKYFVLQVHYGDI-----NHKDCSGVSVHLTRVPQPLIAGMYLMMSVDT---VIPPGEKVVNADISC-
-----------QYKMYPMHVFAYRVHTHHLG-KVVSGYRVRNGQ-WTLIGRQN-------------------PQLP-
-QAFYPVEHPVDVTFGDILAARCVFTGEGRTEATHIGGTSSDEMCNLYIMYYMEAKYALSFMTCTKNVAPDMFRTIP
AEANIPIII*
>P1;temp2
structure:temp2: 74:: 81::
---------------------------------------------------------KVTEGSEQ*
>P1;temp3
structure:temp3: 85:: 97::
-----------------------------------------------------------------------------
---AAKIITHPNFNGN*
>P1;temp4
structure:temp4: 17:: 30::
-----------------------------------------------------------------------------
----------------CKHSMK-YRLSFCRK*
>P1;temp5
structure:temp5: 258:: 269::
-----------------------------------------------------------------------------
-----------------------------------------------------------------------------
----------------------------------------------------------------------------P
DWCTASGTPAS*
>P1;temp6
structure:temp6: 75:: 93::
-----------------------------------------------------------------------------
-----------------------------------------------------------------------------
-----------------------------------------------------------------------------
-----------------------------------------------------KYVPHFKPGKELRDRANIY*
>P1;temp7
structure:temp7: 30:: 47::
-----------------------------------------------------------------------------
-----------------------------------------------------------------------------
-----------------------------------------------------------------------------
-----------------------------------------------------FHPPHI-EIQMLKNGKKIP*
>P1;target
sequence:target:
LLKPSIPKPALPADTC--TMEIRAPDVLIPGQQTTYWCYVTELPDGFPRHHIVMYEPIVTEGNEA-----LV66MEV
FQCAAEFRDHPHFSGPCDSKMKPQRLNFCRH----------------------------VLAAWALGAKAFYYPEEA
GLAFGGPGSSRFLRLEV6YHNPLVIT-GRRDSSGIRLYYTAALRRFDAGIMELGLAYTPVMAIPPQETAFVLTGYCT
DKCTQLALPAS-----GIHIFASQLHTHLTGRKVVTVL-ARDGRETEIVNRDNHYSPHFQEIRMLKKVVSVQ-----
--------------PGDVLITSCTYNTEDRRLATVGGFGILEEMCVNYVHYYPQTQLEL----CKSAVDPGFLHKYF
RLVNR--LL*
Has anyone an idea about the problem? The error is the same if i only
use the 2 first templates for instance.
Thank you, regards.
--
Michel GIORGI
Lab. de Cristallochimie
Faculte de St. Jerome
Marseille, cedex 20, FRANCE