Dear Modeller users,
I'm trying to build a model of a hetero-dimer starting from an X-ray structure. Each monomer have 3 domains (6 in total). An X-ray structure of part of the complex (two domains of each monomer) exist and I want to complete the model with the two missing domains (one for each monomer). I would use the X-ray of homologous proteins for which all 6 domains are available. Which would be the best approach? I want to keep the X-ray part as frozen as possible, and only model the two missing domains from the template (both structure and orientation in the complex).
Thank you very much in advance for any tips
Stefano