Dear Rick and Ryoji,

PageWorks is working on the color slides for our paper. They should
be ready by 1am on Friday. You can collect them at the production 
headquarters at 501 Cambridge St, Cambridge, MA 02141, tel 374 6000,
fax 374 6020. The headquarters are opened non-stop. They are frequently 
ready sooner than in 24 hours so you could check with them and possibly 
collect the slides on Thu. Note that I told them that you would
pick up the slides at the Boston Branch, which is not opened overnight,
so if you want to change this, let them know. I append my order.

I payed for the slides with my Visa card so please send me the bill
so that I can get a refund from my grant. 

I had to do it this way because otherwise I would never get the slides
to Ryoji by Friday. My machine was repaired only late this afternoon.

I will be in Boston over the weekend so if you think we should 
discuss the paper or the slides in person, we can do it.

I only ordered 2 copies of each slide for now because I did not want
to take the risk -- it is always difficult to know how they will turn 
out. You can keep both copies. Once we decide which copies we really
want, and perhaps do some changes, I will order more. 

I have not asked them to do Fig 1. Do you need it or did you already 
do it? I think it is a waste of money to make a print of Figure 1 because 
the journal can easily take the printer printout which is also going to 
be of better quality. Do you have an original printout of the final
Figure 1? What should I do with regard to Figure 1?

There are 10 different color slides:

Fig. 2: (a) As described in the legend. (b) I also prepared a
perpendicular view that I like a lot because it clearly shows the 
central location of the His residues that Ryoji mutated and the 
peripheral location of the other His's. It also shows clearly
the relative positions of all the other charged residues in the
region, which are difficult to see from (a). Figure (a) is
still needed to establish the global position of the charged region,
which is important for the cross-reference to the chymases paper.

Fig. 3: I prepared Figs 3a-d in two styles. Let's see which one you
prefer. One style shows the face of the region, the other style
shows it from the side view and is similar to the mesh figures in our
first paper. Both of them are surprisingly clear and convincing. 
If you cannot identify which one is a,b,c,d, we can do it over the phone.

Once you tell me which figures you want, I will email you some small
changes to the text and legends to reflect the figures better (trivial
things). Martin will want slides with his copy, so if you cannot miss your
copies I will have to order additional copies before you can send 
the paper to him.  This takes only 24 hours (I send the pictures
to PageWorks instantly via a computer).

The slides turned out to be nicer than I expected.

Regards, Andrej

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Pageworks Output Order


PageWorks
Downtown Boston Branch
50 Broad St, Boston, MA 02109
tel: (617) 374 6090
fax: (617) 374 6099
e-mail: jobs@pageworks.com


Customer: Andrej Sali, Assistant Professor
          Box 270 
          The Rockefeller University 
          1230 York Avenue, New York, NY 10021-6399 
          voice (212) 327 7550 
          fax (212) 327 7540 
          e-mail sali@rockvax.rockefeller.edu


To be collected by Dr. Ryoji Matsumoto.


Delivery Method: hold at Downtown Boston


A compressed tar archive sali.tar.Z was ftp-ed to ftp.pageworks.com:pub


Application: Silicon Graphics Inc. RGB files:

1021 -rw-r--r--    1 sali     user      522449 Feb 15 21:40 3a-s.rgb
 806 -rw-r--r--    1 sali     user      412547 Feb 15 21:57 3a.rgb
1017 -rw-r--r--    1 sali     user      520225 Feb 15 21:42 3b-s.rgb
 876 -rw-r--r--    1 sali     user      448212 Feb 15 21:56 3b.rgb
1008 -rw-r--r--    1 sali     user      515587 Feb 15 21:44 3c-s.rgb
 834 -rw-r--r--    1 sali     user      427005 Feb 15 21:55 3c.rgb
1020 -rw-r--r--    1 sali     user      522037 Feb 15 21:45 3d-s.rgb
 807 -rw-r--r--    1 sali     user      413045 Feb 15 21:53 3d.rgb
 553 -rw-r--r--    1 sali     user      282842 Feb 15 23:49 fig2a.rgb
1495 -rw-r--r--    1 sali     user      764993 Feb 16 00:31 fig2b.rgb


Output type: Color slides 35mm, 2 copies each RGB file.


Special instructions: Please orient the slides so that the pictures
are as large as possible. 3a,3b,3c,3d should be oriented in the same
way. 3a-s, 3b-s, 3c-s, 3d-s should be oriented in the same way. Please
make sure that 3a-s, 3b-s, 3c-s, and 3d-s are not too dim.
