Hello! My name is Amanda Maia and I´m a beginner using Modeller. I´m having some trouble finding the answers to my questions on the manual. Could you help me? I have a sequence that aligns (BLAST pdb)with various structures on PDB within an average identity of 35%. Since I need to use as much models as I can get, I´m writing a PIR file with 5 templates. But one (1MDY) has 2 chains (out of 4) that blast quotes (B and E). Do I have to make a PIR line to each one? And a pdb file for each one too? Example: >P1;molde1MDYB structureX:molde1MDYB:FIRST:@:LAST :@:myoD:myoD: 2.00:-1.00 ----------------------------------------------------------------------------------------------------------------------------TTNADRRKAATMR--ERRR------LSKVNEAFETLKRSTSSNPN-----------QRLPKVEILR--------NAIRYIEGLQAL-LRD-------------------------------------* >P1;molde1MDYA structureX:molde1MDYA:FIRST:@:LAST :@:myoD:myoD: 2.00:-1.00 ----------------------------------------------------------------------------------------------------------------------MELKRKTTNADRRKAATMR--ERRR------LSKVNEAFETLKRSTSSNPN-----------QRLPKVEILR--------NAIRYIEGLQAL-LRD-------------------------------------* Thank you in advance! Amanda Maia
Novos endereços, o Yahoo! que você conhece. Crie um email novo com a sua cara @ymail.com ou @rocketmail.com. http://br.new.mail.yahoo.com/addresses