Dear Bharat Lal,
As you see the model sequence is shifted a little bit towards the PDB sequence. Try to add three gaps in the beginning of the PDB alignment like this:
I am modeling the structure of of leptin protein using template as 1AX8 protein with PDB file starting with the 3rd residue .. and I am getting the following error :- Alignment VPIQKVQDDTKTLIKTIVTRINDISHTQSVSSKQKVTGLDFIPGLHPILTLSKMDQPDB IQKVQDDTKTLIKTIVTRINDILDFIPGLHPILTLSKMDQTLAVYQQILTSMPSRNMatch * ***Alignment residue type 18 (V, VAL) does not match pdbresidue type 8 (I, ILE),for align code 1AX8 (atom file 1AX8), pdb residue number "3", chain "A"Please check your alignment file header to be sure you correctly specifiedthe starting and ending residue numbers and chains. The alignment sequencemust match that from the atom file exactly.Another possibility is that some residues in the atom file are missing,perhaps because they could not be resolved experimentally. (Note that Modellerreads only the ATOM and HETATM records in PDB, NOT the SEQRES records.)In this case, simply replace the section of your alignment correspondingto these missing residues with gaps.read_te_288W> Protein not accepted: 1 1AX8
Cn u pls help regarding this ..Bharat
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