Hi:<br>&nbsp;I am using model loop based on initial PDB files, but there is a error:<br><br><br>Traceback (most recent call last):<br>&nbsp; File "model.py", line 41, in ?<br>&nbsp;&nbsp;&nbsp; a.make()<br>&nbsp; File "/usr/lib64/python2.4/site-packages/modeller/automodel/loopmodel.py", line 36, in make<br>&nbsp;&nbsp;&nbsp; automodel.make(self, exit_stage)<br>&nbsp; File "/usr/lib64/python2.4/site-packages/modeller/automodel/automodel.py", line 98, in make<br>&nbsp;&nbsp;&nbsp; self.homcsr(exit_stage)<br>&nbsp; File "/usr/lib64/python2.4/site-packages/modeller/automodel/automodel.py", line 411, in homcsr<br>&nbsp;&nbsp;&nbsp; aln = self.read_alignment()<br>&nbsp; File "/usr/lib64/python2.4/site-packages/modeller/automodel/automodel.py", line 401, in read_alignment<br>&nbsp;&nbsp;&nbsp; aln.append(file=self.alnfile, align_codes=self.knowns+[self.sequence])<br>&nbsp; File "/usr/lib64/python2.4/site-packages/modeller/alignment.py", line 81, in append<br>&nbsp;&nbsp;&nbsp; allow_alternates)<br>IOError: openf______E&gt; Cannot open file : No such file or directory<br><br>