I would like to model a protein which subcellular location is cytosol.
I have found a better template which subcellular location is nuclear.

My queries:
1) What are the considerations or constrainsts to be used to model a cytosol protein using nuclear protein.
   Is is correct to do like this.
2) Is it possible to specify constrainsts to model the environment based on tissue specific.

Thank you in advance for any help