On 2/19/12 11:59 AM, albert wrote: > I am trying to generate loop models with loop protocol in the latest > Modeller verison. It only produced 8 models and stopped, and it said the > following: ... > modeller.parallel.communicator.RemoteError: <type 'ValueError'>: > GA341______E> Sequence identity -999.000 out of range - must be between > 0 and 100. > Seq. ID is read from the model.seq_id variable, which > is obtained automatically from the REMARK lines of > MODELLER-produced PDB files
GA341 takes the sequence identity of the template as a parameter. Are you trying to refine a regular PDB file? If so, you can't use GA341, because Modeller doesn't know the sequence identity. If you're trying to refine a model produced by Modeller, it should work, because that information should be present in the PDB file as a REMARK record.
Ben Webb, Modeller Caretaker