Hi everyone,

I am working on the example given in the modeller tutorial http://salilab.org/modeller/wiki/Missing%20residues. I used the exact same script to generate the sequence file. Instead of the given pdb1qg8.ent , I used the 1qg8.pdb downloaded from www.rcsb.org.  When I ran the script it resulted in the following sequence file (1qg8.seq)


>P1;1qg8
structureX:1qg8:   2 :A:+238 :A:MOL_ID  1; MOLECULE  SPORE COAT POLYSACCHARIDE BIOSYNTHESIS PROTEIN SPSA; CHAIN  A; ENGINEERED  YES; OTHER_DETAILS  GLYCEROL MAGNESIUM:MOL_ID  1; ORGANISM_SCIENTIFIC  BACILLUS SUBTILIS; GENE  SPSA; EXPRESSION_SYSTEM  ESCHERICHIA COLI; EXPRESSION_SYSTEM_STRAIN  BL 21: 1.50: 0.16
PKVSVIMTSYNKSDYVAKSISSILSQTFSDFELFIMDDNSNEETLNVIRPFLNDNRVRFYQSDISGVKERTEKTR
YAALINQAIEMAEGEYITYATDDNIYMPDRLLKMVRELDTHPEKAVIYSASKTYHLNDIVKETVRPAAQVTWNAP
CAIDHCSVMHRYSVLEKVKEKFGSYWDESPAFYRIGDARFFWRVNHFYPFYPLDEELDLNYITEFVRNLPPQRNC
RELRESLKKLGMG*

In the field 5, it says +238 whereas in the tutorial, in the resulting 1qg8.seq file it shows 256. Have I done any mistake? Would the above sequence file work just as fine as the one shown in the tutorial?

Thank you

Sajeewa Dewage