Hi, Modeller caretakers...
Recently, i am trying to introduce HETATM into my built model, however i have encountered a problem, which is 631E:
"No aligned template residues for BLK residue; number of templates: @I1 Make sure that each BLK residue in your target sequence is aligned with your templates. (This can also be caused by a lack of structural information. Make sure you are using a PIR, not FASTA, alignment file, and that the template headers use 'structureX' not 'sequence'.)"
and this is my alignment:
>P1;2ZWRA structureX:2ZWR.pdb: 1 :A:+202 :A:MOL_ID 1; MOLECULE METALLO-BETA-LACTAMASE SUPERFAMILY PROTEIN; CHAIN A, B; ENGINEERED YES:MOL_ID 1; ORGANISM_SCIENTIFIC THERMUS THERMOPHILUS; ORGANISM_TAXID 300852; STRAIN HB8; GENE TTHA1623; EXPRESSION_SYSTEM ESCHERICHIA COLI; EXPRESSION_SYSTEM_TAXID 562; EXPRESSION_SYSTEM_VECTOR_TYPE PLASMID; EXPRESSION_SYSTEM_PLASMID PET11A: 2.20: 0.19 MRVFPVTLGPLQENAYLVETG-EGPVLIDPGDEPEKLLALFQTTGLIPLAILLTHAHFDHVGAVAPLVEALDLPV YLHP-----L-DLPLYEGADLAARAWGLAIPKPPLPVRPLEEGMRLFGFQVLHLPGHSPGHVAFYDPEGAQVFSG DLLFRGSVGRYDLPGADPKALFASLK-RLLSLPPETRVHPGHGPGTTLGLEARTNPFL..*
>P1;Metallo-beta-lactamase sequence:Metallo-beta-lactamase: :212 : : ::: 0.00: 0.00 M-FKQIPLGPIQTNAYVLYNDDKEAVIFDPGGDAEALITWLKREQLTPLAILLTHAHFDHIGAVDAVRDTFSIPV YLHTKERHWLEDPALNGSSRLTGRPITTAKPADHLLTNEKSLTIGTFTFSVFHTPGHSPGSVSYYYQKEAVLFSG DVLFQQSIGRTDLRGGDHTLLLASIHNKILPLPERTIVASGHGPLTTIGQEMDHNPFLTGY--..*
Your suggestion is much more appreciated... Thank you