Hi,


I would like to model missing portion of a solved protein structure by using two different templates. I know that is pretty straightforward to use multiple templates, but I would like to include restraints based on the first entry only. Basically I would like to keep the first solved structure as it is, and just move the residues lining the missing portions, while using the second structure as a 'flexible' template. How can I define that I want the restraints based only on the first template? I tried to build a chimera, but I have a bunch of clashes if I do that.


Thanks,


Paola


Paola Bisignano, Ph.D.
postdoctoral research associate
Department of Pharmaceutical Chemistry
Cardiovascular Research Institute
University of California, San Francisco