Dear Modellers,
I try to model my target protein on several templates (a big one and 6 very small ones), moreless as it is indicated in question 4 of the FACs.
Unfortunately the program ends in error when checking the alignements, with the following error message:
################### fit2xyz_E> number of equivalent positions < 3: 0 recover__> MODELLER_STATUS >= STOP_ON_ERROR: 1 1 ###################
My alignement file looks like this:
>P1;temp1 structure:temp1: 45:: 356:: NECLGTIGPVTPLDASDFALDIRMPGVT-PKESDTYFCMSMRLPVD-EEAFVIDFKP--------RASMDTVHHMLL
FGC----------------------------N--MPSSTGSYWFCDEGTCTDKAN----ILYAWARNAPPTRLPKGV
GFRVGGETGSKYFVLQVHYGDI-----NHKDCSGVSVHLTRVPQPLIAGMYLMMSVDT---VIPPGEKVVNADISC-
-----------QYKMYPMHVFAYRVHTHHLG-KVVSGYRVRNGQ-WTLIGRQN-------------------PQLP-
-QAFYPVEHPVDVTFGDILAARCVFTGEGRTEATHIGGTSSDEMCNLYIMYYMEAKYALSFMTCTKNVAPDMFRTIP
AEANIPIII*
>P1;temp2 structure:temp2: 74:: 81:: ---------------------------------------------------------KVTEGSEQ*
>P1;temp3 structure:temp3: 85:: 97:: -----------------------------------------------------------------------------
---AAKIITHPNFNGN*
>P1;temp4 structure:temp4: 17:: 30:: -----------------------------------------------------------------------------
----------------CKHSMK-YRLSFCRK*
>P1;temp5 structure:temp5: 258:: 269:: -----------------------------------------------------------------------------
-----------------------------------------------------------------------------
----------------------------------------------------------------------------P
DWCTASGTPAS*
>P1;temp6 structure:temp6: 75:: 93:: -----------------------------------------------------------------------------
-----------------------------------------------------------------------------
-----------------------------------------------------------------------------
-----------------------------------------------------KYVPHFKPGKELRDRANIY*
>P1;temp7 structure:temp7: 30:: 47:: -----------------------------------------------------------------------------
-----------------------------------------------------------------------------
-----------------------------------------------------------------------------
-----------------------------------------------------FHPPHI-EIQMLKNGKKIP*
>P1;target sequence:target: LLKPSIPKPALPADTC--TMEIRAPDVLIPGQQTTYWCYVTELPDGFPRHHIVMYEPIVTEGNEA-----LV66MEV
FQCAAEFRDHPHFSGPCDSKMKPQRLNFCRH----------------------------VLAAWALGAKAFYYPEEA
GLAFGGPGSSRFLRLEV6YHNPLVIT-GRRDSSGIRLYYTAALRRFDAGIMELGLAYTPVMAIPPQETAFVLTGYCT
DKCTQLALPAS-----GIHIFASQLHTHLTGRKVVTVL-ARDGRETEIVNRDNHYSPHFQEIRMLKKVVSVQ-----
--------------PGDVLITSCTYNTEDRRLATVGGFGILEEMCVNYVHYYPQTQLEL----CKSAVDPGFLHKYF
RLVNR--LL*
Has anyone an idea about the problem? The error is the same if i only use the 2 first templates for instance. Thank you, regards.
-- Michel GIORGI Lab. de Cristallochimie Faculte de St. Jerome Marseille, cedex 20, FRANCE