The alignment probably aligns residues in known structures that are quite far in space, requiring a lot of memory for the splines to describe the corresponding multi-modal gaussian functions (this is probably the same problem that was discussed here last week or so). The solution is to improve the alignment (by only aligning residues that can be aligned close in space; close meaning about 5A or better; and not aligning residues that are further apart); possibly, MODELLER6v1 is a little less stingy with memory assignment for splines, so maybe it would work on your current alignment.
Best wishes, Andrej
-- Andrej Sali, Associate Professor The Rockefeller University, 1230 York Avenue, New York, NY 10021-6399 tel +1 212 327 7550; lab +1 212 327 7206 ; fax +1 212 327 7540 e-mail sali@rockefeller.edu; http://salilab.org
-----Original Message----- From: owner-modeller_usage@salilab.org [mailto:owner-modeller_usage@salilab.org] On Behalf Of Luca Settimo Sent: Monday, February 18, 2002 9:45 AM To: 'Modeller Usage List' Subject: mkspline_E
Dear modeller, I was using still modeller 4 (just to complete one work that I was doing) I have one problem, I m building a model using 7 templates (I made a structural alignment that I am using to build my model). Everything works fine... except that for one situation (so my input file top and ali are correspondent, except the sequence that I have to model... like you can immagine)
and I have this message:
mkspline_E> too many parameters, increase MPRMCNS
if I remove one template it works fine...
what does it mean this message? how can I still use 7 templates?
Luca
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Luca Settimo Molecular Structures and Biocomputing Department of Biochemistry and Pharmacy Åbo Akademi University Tykistökatu 6A 20520 Turku Finland phone: +358 (0) 2 2154600 fax: +358 (0) 2 2153280 mobile phone: +358 50 5238418 mailto:lsettimo@abo.fi http://www.abo.fi/~lsettimo