Thank you very much for your reply.
Yes, I am trying to determine which of the 4 sequences is the best.
I will make a model for each of the 4 templates then.

Thank you.

Best wishes,
Susana

On Wed, Feb 29, 2012 at 6:31 PM, Modeller Caretaker <modeller-care@salilab.org> wrote:
On 2/29/12 10:01 AM, Susana Tomasio wrote:
   I am trying to search for the best template for my target molecule,
   and therefore I created a file in PIR format with my sequences and then
   I ran build_profile.py. I got the following error in my log file:

build_profile is designed to search through a sequence database (e.g. all sequences from PDB or UniProt) to find matches for a single query sequence. You've built a database of only 4 sequences. This is not nearly large enough for build_profile to be able to determine accurate statistics. What are you trying to do? If you want to determine which of the 4 sequences is the best template, one possible solution is simply to build models for each of the 4 templates and then assess them.

       Ben Webb, Modeller Caretaker
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