Hi Arun how many residues in each chain total <div>and how many residues are missing in each chain </div><div>that need to be modeled? Best, Joel 🚀<br><br>On Thursday, August 31, 2023, Ajasja Ljubetič via modeller_usage &lt;<a href="mailto:modeller_usage@salilab.org">modeller_usage@salilab.org</a>&gt; wrote:<br><blockquote class="gmail_quote" style="margin:0 0 0 .8ex;border-left:1px #ccc solid;padding-left:1ex"><div dir="ltr"><div dir="ltr">I think the only trick is not forgetting the chain break in the ali file:<div><a href="https://salilab.org/modeller/manual/node29.html" target="_blank">https://salilab.org/modeller/<wbr>manual/node29.html</a><br></div><div><br></div><div>Best,</div><div>Ajasja</div><div><br></div><div>PS: It&#39;s a very broad question, so I can only give a broad reply.</div></div></div><br><div class="gmail_quote"><div dir="ltr" class="gmail_attr">On Thu, 31 Aug 2023 at 09:12, Arun Dhanasekaran via modeller_usage &lt;<a href="mailto:modeller_usage@salilab.org" target="_blank">modeller_usage@salilab.org</a>&gt; wrote:<br></div><blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left:1px solid rgb(204,204,204);padding-left:1ex"><div dir="ltr">i tried a build a homology model of a heterodimer protein,since it contains missing residue i have to model that. But i am only familiar with single chain homology modelling and fro multi chain i am getting many errors like in greeting ali flie. and from tutorial also i am not getting everything clearly. can i get a detailed explantiojn on multi chain modelling<div><br></div></div>
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