dear all; I am going to build a model based on a multiple subunit membrane chanel(a five subunit homopantameric one); it is suggested that I build these kind of models in a "successive" manner, meaning:keeping the template multisubunit and running modeller with the target sequence; then again keeping the template subunits (all) along with the first and second target sequences; then again having template in hand with the dimer made, building the third subunit and so on... somebody guide me:1- what is the benefit of this successive step modelling? 2- how should I perform this? does anybody have an example file to show me how to add previous models to the current project?

thanks. adak N.

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