Hello,

Actually I want to build a homology model of a human homotrimeric protein from a FASTA sequences. After BLAST I got a template which is 91% identical but it is originated from Mouse organism. And it is the Cryo-EM structure. >From there, I build a single monomeric chain of that human protein by Homology modelling. But it doesn't contain any biological assembly or asymmetric unit. So that, i am unable to build its trimeric structure. so, can you help me in two sections; 1) how to build its homotrimeric structure? 2) is there any way to generate the biological assembly coordinates from homology protein? Thanks in advance. Best regards.

Sourav PalSenior Project FellowResearch Group of Dr. Arindam TalukdarCSIR-Indian Institute of Chemical BiologyJadavpur, Kolkata, India