mutate protion dimer
hello All
I have a protein dimer and I have to insert amino acid rsidues in both monomer at same palce. Is it possible to do with Modller without affecting interaction between protein in crystal structure?
With regards
Sangita Kachhap SRF BIC,IMTECH CHANDIGARH
______________________________________________________________________ सूक्ष्मजीव प्रौद्योगिकी संस्थान (वैज्ञानिक औद्योगिक अनुसंधान परिषद) Institute of Microbial Technology (A CONSTITUENT ESTABLISHMENT OF CSIR) सैक्टर 39 ए, चण्डीगढ़ / Sector 39-A, Chandigarh पिन कोड/PIN CODE :160036 दूरभाष/EPABX :0172 6665 201-202
On 12/07/2012 10:36 PM, Sangita Kachhap wrote: > I have a protein dimer and I have to insert amino acid rsidues in both monomer > at same palce. Is it possible to do with Modller without affecting interaction > between protein in crystal structure?
Sure, that should be straightforward (whether it affects the interaction depends on how big the insertion is and how close it is to the interface). Just use your original dimer as the template and build a model using an alignment that looks something like
template AAAAA--AAAAA/AAAAA--AAAAA* model AAAAABBAAAAA/AAAAABBAAAAA*
where A represents the original sequence of your dimer, / the chain break, and B the inserted residues.
Ben Webb, Modeller Caretaker
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Modeller Caretaker -
Sangita Kachhap