Modeling a Chimera
Hi dears,
I am a starting model buiter and i´d like to know how i can make a model like this :
I have a bifuncional enzime with two domains. But in the PDB i have as a model different structures : One for domain A and other to domain B.
And i´d like to build a model with this two domains together. It is not a dimer, it a single enzime with two diferente domains. And i´d like to know tto, what i will do with the linker resudues between this both domains !!
I anybody can help me, please be detalist cause i am a start user i am using Modeller 8v0
thanks a Lot
Marcos Tadeu dos Santos Undergratuated Student UNICAMP - BRAZIL
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Toty Santos wrote: > I have a bifuncional enzime with two domains. But in the PDB i have as a > model different structures : One for domain A and other to domain B. > > And i´d like to build a model with this two domains together. It is not a > dimer, it a single enzime with two diferente domains. And i´d like to know > tto, what i will do with the linker resudues between this both domains !!
See http://salilab.org/modeller/FAQ.html#1
In your model sequence, you may need to use the '/' character to denote a chain break between the two domains.
To ensure the correct orientation of the two domains, you will need to add additional information (e.g. a template sequence that partially overlaps the two, or manual distance restraints from experiments or other data).
> I anybody can help me, please be detalist cause i am a start user > i am using Modeller 8v0
You should use Modeller 8v2 instead, which fixes some bugs in 8v0.
Ben Webb, Modeller Caretaker
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Modeller Caretaker -
Toty Santos