modelling coordinate of missing residues
Hello all I have a pdb file and coordinate of 13 amino acid (N-terminal arm)are missing in this pdb file.
Is it possible to generate coordinte of these missing residue?
I have a pdb file of other protein of same family in this pdb file N-terminus is random coil and turn and in my query pdb N-terminus is random coil and 3-10 helix.
I have predicted the secondary structure for missing residues they are random coil and sequence identity is of query with template except N-terminal is 68%.
So is it possible to model a protein of query sequence using above mension known pdb file?
Sangita Kachhap JRF BIC,IMTECH CHANDIGARH
On 4/25/11 11:49 PM, Sangita Kachhap wrote: > I have a pdb file and coordinate of 13 amino acid (N-terminal arm)are missing in > this pdb file. > > Is it possible to generate coordinte of these missing residue?
Sure; see http://salilab.org/modeller/wiki/Missing%20residues
However, the coordinates are unlikely to be correct, since your missing residues are at the terminus. Since only one end is anchored, there's a lot of conformational space to explore. Thus, I would suggest you build a model without this terminal region.
Ben Webb, Modeller Caretaker
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Modeller Caretaker
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Sangita Kachhap