Hello Dr. Owen. I am sorry I did not explain well. I am not able to compute H-bonds using Swiss-PDBViewer and I am getting "Unrealistic B-factor 0.0" in the log file of the mutant protein. (I had generated the mutant protein using the MUTATE_MODEL command of MODELLER.) Any suggestions will be appreciated.

> Hi Jaimin, > > What do you mean by compute H-bonds? If you mean you want to add hydrogen > atoms and calculate some kind of energy i'd suggest using a molecular > mechanics program such as CHARMM. Hope this is of help! > > Owen > > >

Sincerely, Jaimin Shah M.Sc. (Biotechnology) School of Biosciences & Bioengineering I.I.T. Bombay