Hi, Ben Thank you first. The .top file is: INCLUDE

SET OUTPUT_CONTROL = 1 1 1 1 1 SET ATOM_FILES_DIRECTORY = ' ./ ' SET ALNFILE = '1bl8-kv14.ali ' SET KNOWNS = '1bl8.pdb' SET SEQUENCE = 'kv14' SET STARTING_MODEL = 1 SET ENDING_MODEL = 10

CALL ROUTINE = 'model'

And the .ali is:

>P1;1bl8.pdb structureX: 1bl8.pdb: 23: A: 119: D: : : : ALHWRAAG AATVLLVIVLLAGSYLAVLAERGAPGAQLITYPRALWWSVETATTVGYGDLYPVTLWGRC VAVVVMVAGITSFGLVTAALATWFVGREQ/ ALHWRAAG AATVLLVIVLLAGSYLAVLAERGAPGAQLITYPRALWWSVETATTVGYGDLYP VTLWGRCVAVVVMVAGITSFGLVTAALATWFVGREQ/ ALHWRAAGAATVLLVIVLLAGSYLAVLAERGAPGAQLITYPRALWWSVETATTVGYGDLYP VTLWGRCVAVVVMVAGITSFGLVTAALATWFVGREQ/ ALHWRAAG AATVLLVIVLLAGSYLAVLAERGAPGAQLITYPRALWWSVETATTVGYGDLYP VTLWGRCVAVVVMVAGITSFGLVTAALATWFVGREQ/*

>P1;kv14 sequence: kv14: 474: A: 570: D: : : : RASMRELGLLIFFLFIGVILFSSAVYFAEADEPTTHFQSIPDAFWWAVVTMTTVGYG DMKPITVGGKIVGSLCAIAGVLTIALPVPVIVSNFNYFYH/ RASMRELGLLIFFLFIGVILFSSAVYFAEADEPTTHFQSIPDAFWWAVVTMTTVGYG DMKPITVGGKIVGSLCAIAGVLTIALPVPVIVSNFNYFYH/ RASMRELGLLIFFLFIGVILFSSAVYFAEADEPTTHFQSIPDAFWWAVVTMTTVGYG DMKPITVGGKIVGSLCAIAGVLTIALPVPVIVSNFNYFYH/ RASMRELGLLIFFLFIGVILFSSAVYFAEADEPTTHFQSIPDAFWWAVVTMTTVGYG DMKPITVGGKIVGSLCAIAGVLTIALPVPVIVSNFNYFYH/ *

And in .log file there are ten models from B99990001 to B99990010.

Many thanks

BinBin