What is the length of the target protein, which you are calling a Difficult one. Your aln file is not found. Attach your align2d.py or salign.py respectively, if you are using single or multiple templates for modeling. Also attach your target sequence target.seq file.

Good Luck, Ashish

Ashish Runthala, Lecturer, Structural Biology Cell, Biological Sciences Group, Birla Institute of Technology & Science, Pilani, Rajasthan - 333031

From: ranu sharma ranusharma09@gmail.com Subject: [modeller_usage] problem in doing difficuly modeling using modeller 9v8 To: modeller_usage@salilab.org Message-ID: AANLkTikhoQUvqSQA1GkKauo9=XctiXy2v27mX-LWrtYZ@mail.gmail.com Content-Type: text/plain; charset="iso-8859-1"

Hello Sir/Madam,

Good Afternoon. I am facing problem while doing Difficult modelling for my protein. It is showing the following error. I am attaching the .aln file, my .pdb and model.py file for your reference. Hope to get a reply from you soon.

Thanks & Regards.

*ERROR:*

C:\Program Files\Modeller9v8\examples\ranu_basic\C4H>mod9v8 model.py 'import site' failed; use -v for traceback Traceback (most recent call last): File "model.py", line 9, in ? a.make() File "C:\Program Files\Modeller9v8\modlib\modeller\automodel\automodel.py", li ne 98, in make self.homcsr(exit_stage) File "C:\Program Files\Modeller9v8\modlib\modeller\automodel\automodel.py", li ne 424, in homcsr self.check_alignment(aln) File "C:\Program Files\Modeller9v8\modlib\modeller\automodel\automodel.py", li ne 406, in check_alignment aln.check() File "C:\Program Files\Modeller9v8\modlib\modeller\alignment.py", line 200, in check self.check_structure_structure(io=io) File "C:\Program Files\Modeller9v8\modlib\modeller\alignment.py", line 209, in check_structure_structure return f(self.modpt, io.modpt, self.env.libs.modpt, eqvdst) _modeller.ModellerError: check_a_282E> Unable to read structural information for alignment sequence: 1 2fdvA This command requires structures.