Hello I am working with a protein having three domains and two hinge between them. I have x-ray structure of protein. now, I want to keep the domains rigid and then apply some experimental data(EPR distances) in order to have new conformation. I use rigid body command to keep domains rigid and then use restraints to define the distances. But modeller does not apply the distance. What is the problem and how I can apply such restraints.
Thanks Reza
On 2/9/12 1:46 AM, Ahmadreza Mehdipour wrote: > I am working with a protein having three domains and two hinge between > them. I have x-ray structure of protein. now, I want to keep the domains > rigid and then apply some experimental data(EPR distances) in order to > have new conformation. I use rigid body command to keep domains rigid and > then use restraints to define the distances. But modeller does not apply > the distance. What is the problem and how I can apply such restraints.
What you're doing sounds fine. So you probably have an error in your script somewhere - if you can post it, it should be easy to see where the error is.
Ben Webb, Modeller Caretaker
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Ahmadreza Mehdipour -
Modeller Caretaker