subarna thakur wrote: > I facing a problem for building a model of a hetrotetrameric protein > with 4 chain. Chain A and C are similar and have 423 aminoacids while > chain B and D have 504 amino acids. The template I am taking is also a > hetero tetrameric protein. Can any body please tell me how to write the > header of the alignment file of the template as well as my target > protein. The example given in the manual isnt quite helpful as it shows > the case where there is just two chain.

Building a model with four chains will be exactly the same as building a model with two chains (except, obviously, you will have three chain breaks rather than just one in each sequence) so the example should be applicable. What, specifically, is the problem you're encountering?

Ben Webb, Modeller Caretaker