Hi,

I have been trying to MODEL a protein with distance restraints using MODELER version-12 with InsightII . Here I am attaching my .rsr file, and the below are the problems i got in my trials....

MODELLER12 VERSION: USER FORMAT R 3 1 1 21 2 2 0 7.1000 0.3387 SG:140 SG:222 R 3 1 1 21 2 2 0 16.0000 0.3387 CA:139 CA:248 R 3 1 1 22 2 2 0 17.0000 0.3387 CA:139 CA:248 R 3 1 1 21 2 2 0 17.0000 0.3387 CB:139 CB:248 R 3 1 1 22 2 2 0 18.0000 0.3387 CB:139 CB:248 R 3 1 1 21 2 2 0 16.0000 0.3387 CA:139 CA:251 R 3 1 1 22 2 2 0 17.0000 0.3387 CA:139 CA:251 R 3 1 1 21 2 2 0 16.5000 0.3387 CB:139 CB:251 R 3 1 1 22 2 2 0 17.5000 0.3387 CB:139 CB:251 R 3 1 1 21 2 2 0 19.6000 0.3387 CA:139 CA:252 R 3 1 1 22 2 2 0 20.6000 0.3387 CA:139 CA:252 R 3 1 1 21 2 2 0 21.5000 0.3387 CB:139 CB:252 R 3 1 1 22 2 2 0 22.5000 0.3387 CB:139 CB:252 R 3 1 1 21 2 2 0 13.3000 0.3387 CA:139 CA:249 R 3 1 1 22 2 2 0 15.3000 0.3387 CA:139 CA:249 R 3 1 1 21 2 2 0 11.4000 0.3387 CB:139 CB:249 R 3 1 1 22 2 2 0 13.4000 0.3387 CB:139 CB:249 R 3 1 1 21 2 2 0 15.0000 0.3387 CA:139 CA:250 R 3 1 1 22 2 2 0 17.0000 0.3387 CA:139 CA:250 R 3 1 1 21 2 2 0 15.3000 0.3387 CB:139 CB:250 R 3 1 1 22 2 2 0 17.3000 0.3387 CB:139 CB:250 R 3 1 1 21 2 2 0 10.0000 0.3387 CA:117 CA:292 R 3 1 1 21 2 2 0 6.00000 0.3387 N:117 N:292 R 3 1 1 27 2 2 0 8.00000 0.3387 CB:117 CG:208 R 3 1 1 27 2 2 0 10.0000 0.3387 CB:117 CG:208 R 3 1 1 27 2 2 0 8.00000 0.3387 CB:117 CG:211 R 3 1 1 27 2 2 0 10.0000 0.3387 CB:117 CG:211

1) Modeler is not recognising the "SG" (first line) or "CG" (last four lines) atoms, when i am trying to run the MODELER program.

2) If I remove these lines the program is getting CORE dumped.

and also I didnt under stand when to use 21, 22, 27 and 30 in distance restraints.

can you please advice me what might be the reason, and what should i do....

regards, Praveen