Re: modeller running under IRIX6.3

[Azat Badretdinov <azat>]

Hi, Pedro!

Sure, you can. It is standard example in the manual.

Specify whatever sequences you want as template, put them into .seq file
and you will get a result. One comment: I would modify the automatic
alignment when the wild type was as a template. The alignment program
will probably align all the residues of the mutant with their
counterparts in wild type. I would de-align several residues flanking
the gap.

Please refer to the manual for more details.

Regards,

Azat.

Dr P.A. Reche wrote:
> 
>         Dear Azat,
> 
> I have made a mutant by removing 7 residues from the original protein
> which structure is known. The deletion is in a loop conecting two
> b-strands. What I what to do is to get a model for this new mutant using
> the original structure as template? Could I improve the model using also
> as template the structures of other proteins related to the original one
> and which are trying to emulate by making my mutants?
> Thanks in advance.
> 
> Sincerely,
> 
> Pedro Reche
> 
> =================================================================================
> Dr. Pedro Antonio Reche Gallardo
> Dept. of Biochemistry, U. of Cambridge,
> 80 Tennis Court Road, CB2 1GA, Cambridge, England, UK
> Phone: +44 (0)1223 333662
> Fax: +44 (0)1223 333661
> , 
> http://www-ccmr-nmr.bioc.cam.ac.uk/~par

--
- Dr. Azat Badretdinov 
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