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[modeller_usage] regarding N terminus region
To : "modeller_usage" <modeller_usageATsalilab.org >
Subject : [modeller_usage] regarding N terminus region
From : "simran jeet" <jeet_simATrediffmail.com >
Date : 17 Oct 2007 10:24:44 -0000
I have a protein sequence whose N terminus region does not matches with the template. Is it neccessary that N and C terminus regions of a protein should be fixed for homology modeling? The n terminus region contains 12 residues. There are long gaps also within the alignment. Can these regions be modelled by loop modelling? I am also attaching the alignment file.
>P1;1o98A
structureX:1o98.pdb: 2 :A:+509 :A:undefined:undefined:-1.00:-1.00
------------SKKPVALIILDGFALRDETYGNAVAQANKPNFDRYWNE-YPHTTLKACGEAVGLPE-GQMGNS
EVGHLNIGAGRIVYQSLTRINIAIREGEFDRNETFLAAMNHVKQHGTSLHLFGLLSDGGVHSHIHHLYALLRLAA
KEGVKRVYIHGFLDGRDVGPQTAPQYIKELQEKIKEYGVGEIATLSGRYYSMDRDK------RWDRVEKAYRAMV
YGEGPTYRDPLECIEDSYKHG--IYDEFVLPSVIVREDGRPVATIQDNDAIIFYNFRPDRAIQISNTFTNEDFRE
FDRGPKHPKHLFFVCLTHFSETVAGYVAFKPTNLDNTIGEVLSQHGLRQLRIAETEKYPHVTFFMSGGREEEFP-
GEDRILINSPKVPTYDLKPEMSAYEVTDALLKEIEADKYDAIILNYANPDMVGHSGKLEPTIKAVEAVDECLGKV
VDAILAKGGIAIITADHGNADEVLTPDGK------------PQTAHTTNPVPVIVTKKGIKLRD-------GGIL
GDLAPTMLDLLGLPQPKEMTGKSLIV---*
>P1;IPGM
sequence:IPGM: : : : :::-1.00:-1.00
MSALSLQLHTALPRRKLLLVVMDGVGIGPGDEYDAVHVAKTPFIDSMCADAKHFRSVCAHGTAVGLPTDADMGNS
EVGHNALGSGRVVLQGASLVDDAIKTGEIFTSDGYRYLHGAFSQPGRTLHLIGLLSDGGVHSRDNQLYEIINHAS
SNGAKRIRLHVLYDGRDVPDKSSFKFTDDLESVLEKARAKGCDARIASGGGRMFVTMDRYEADWSVVERGWRAQV
LGEARAFASAGEAIKTFRKEDPNVSDQYYPPFIISDVDGKAIGPIEDGDAVLCFNFRGDRVIEMSRAFEEEDFDK
FNRVRVPKVRYAGMMRYDGDLGIPNNFLVPPPRLSRTSEEYLVGSGCNIFACSETQKFGHVTYFWNGNRSGKLDE
EHETFFEIPSDRVQFNEKPLMKSKEITEAAIEALKSGKYDVVRINFPNGDMVGHTGDLAATVTAVEAVDASLQRL
KEAVDAVNGVFLITADHGNSDDMAQRDKKGKPILGKDGKVLPLTSHTLAPVPVFIGGAGLDARVQMRTGLPKAGL
ANVTATFLNLMGFTAPTDYEPSLIEVIPN*