Dear Modeller users, I try to model homedimer with few point mutations using hometrimer as a template. In the alignment file I specified to use only chain A and chain B from the template, but the out put dimer model has different monomer-monomer interface than template. I used standard script( http://salilab.org/modeller/9v4/manual/node28.html) and the alignment file: >P1;inv3HD sequence:inv3HD:1:.: 331 ::MODELLING PROTEIN:undefined:: MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLA LHAPSQIVAAIKSRADQLGASVVVPMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVP ALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTLNQIQPIAER EGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAIAESGLRVGADISVVGYDDTEDSSCYIPPLTT IKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLA\MKPVTLYDVAEYAGVSYQTVSRVVNQAS HVSAKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVPMVE RSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHEDGTRLGVE HLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTLNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLV ANDQMALGAMRAIAESGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGN QLLPVSLVKRKTTLA* >P1;1EFA structureX:1EFA:2:A: 329 :.:LAC REPRESSOR:undefined:: -KPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLA LHAPSQIVAAIKSRADQLGASVVVSMVERSGVEACKTAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVP ALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAER EGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYIPPLTT IKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLA* >P1;1EFA structureX:1EFA:2:B: 329 :.:LAC REPRESSOR:undefined:: -KPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLA LHAPSQIVAAIKSRADQLGASVVVSMVERSGVEACKTAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVP ALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAER EGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYIPPLTT IKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLA* Thank you very much in advance for any tips -Manjunatha Karpenahalli --- On Tue, 29/7/08, Modeller Caretaker <> wrote: From: Modeller Caretaker <> |