[modeller_usage] clockwise pentamer to anticlockwise model
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Subject: [modeller_usage] clockwise pentamer to anticlockwise model
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Date: Fri, 19 Sep 2008 16:32:04 -0500
Priority: normal
Dear colleagues,
I want to model a pentamer. I have two templates. One of the templates is arranged anti-clockwise in the order ABCDE. The other template runs clockwise (AEDCB) and has identical chains. Thus if I overlap the two templates, subunit B overlaps with subunit E and so on.
I want to do modelling in 2 steps.
a. I want to get an initial anticlockwise model (ABCDE) from clockwise template (identical chains AEDCB). b. The model obtained from step 1 will be overlapped with the anticlockwise template to build some missing residues.
I tried to get through step a by
1. Changing the alignment file 2. by changing chain identification number in the template 3. by changing the order of subunits arranged in the template
Each time, I end up with the same clockwise model.
Thanks in advance for any kind of suggestion in this regard.