Subject: [modeller_usage] Modeling with multiple templates
From: sdh <>
Date: Sat, 12 Jun 2010 15:47:41 +0200
Hello all!
I want to model a big fibrous protein using several templates. The
templates do not overlap (gap is no longer then 1-3 residues) or overlap
over few residues.
I found the following sentence in the FAQ: "If no additional information
is available about the relative orientation of the two domains the
resulting model will probably have an incorrect relative orientation of
the two domains when the overlap between A and B is non-existing or
short. To obtain satisfactory relative orientation of modeled domains in
such cases, orient the two template structures appropriately before the
modeling."
My questions are: how precisely the templates have to be oriented? Would
be cursory placing enough?
How can I order the templates by using some simple constraints instead
of orienting the templates?
Thanks for help,
sdh