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Re: [modeller_usage] DOPE score for multi chain protein



On 9/28/10 9:39 AM, Omar Piña wrote:
I am modelling a tetrameric protein (ion channel). I want to evaluate
the models, but  I have read that the DOPE assesment is not good for
multi chain proteins
(http://www.salilab.org/modeller/9v6/manual/node242.html).

That's not correct - we simply have no benchmark data to prove whether it is good or bad. It has only been thoroughly tested on single chains. It *appears* to function rather well on multichain models, however.

Then, how is
the best way to evaluate them? Each chain should be evaluated
independently? Should I take only the best molpdf score?

You certainly should not use the molpdf score - the Modeller scoring function does a great job for optimization, but it is not designed to discriminate good models from bad models at assessment time.

	Ben Webb, Modeller Caretaker
--
             http://www.salilab.org/modeller/
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