[modeller_usage] problem of build_profile

[Bishwajit Das <>]

When i run this program showing an error

from modeller import *
log.verbose()
env = environ()

#-- Prepare the input files

#-- Read in the sequence database
sdb = sequence_db(env)
sdb.read(seq_database_file='pdb95.fsa', seq_database_format='FASTA',

         chains_list='ALL', minmax_db_seq_len=(1, 40000), clean_sequences=True)

#-- Write the sequence database in binary form
sdb.write(seq_database_file='pdb95.bin', seq_database_format='BINARY',

          chains_list='ALL')

#-- Now, read in the binary database
sdb.read(seq_database_file='pdb95.bin', seq_database_format='BINARY',
         chains_list='ALL')

#-- Read in the target sequence/alignment

aln = alignment(env)
aln.append(file='toxin.ali', alignment_format='PIR', align_codes='ALL')

#-- Convert the input sequence/alignment into
#   profile format
prf = aln.to_profile()

#-- Scan sequence database to pick up homologous sequences
prf.build(sdb, matrix_offset=-450, rr_file='${LIB}/blosum62.sim.mat',
          gap_penalties_1d=(-500, -50), n_prof_iterations=5,
          check_profile=False, max_aln_evalue=0.01, gaps_in_target=False)

#-- Write out the profile
prf.write(file='buildprofile.prf', profile_format='TEXT')

#-- Convert the profile back to alignment format
aln = prf.to_alignment()

#-- Write out the alignment file

aln.write(file='buildprofile.ali', alignment_format='PIR')


could not open the file pdb_95.fsa

where i get this file??

-- 



BISHWAJIT DAS
+91-9433237920
   KOLKATA