[Date Prev][Date Next][Thread Prev][Thread Next][Date Index][Thread Index]

[modeller_usage] Modeller error, 631E



Hi, Modeller caretakers...

Recently, i am trying to introduce HETATM into my built model, however i have encountered a problem, which is 631E:
"No aligned template residues for BLK residue;
number of templates: @I1
Make sure that each BLK residue in your target
sequence is aligned with your templates.
(This can also be caused by a lack of structural
information. Make sure you are using a PIR, not
FASTA, alignment file, and that the template
headers use 'structureX' not 'sequence'.)" 


and this is my alignment:

>P1;2ZWRA
structureX:2ZWR.pdb:   1 :A:+202 :A:MOL_ID  1; MOLECULE  METALLO-BETA-LACTAMASE SUPERFAMILY PROTEIN; CHAIN  A, B; ENGINEERED  YES:MOL_ID  1; ORGANISM_SCIENTIFIC  THERMUS THERMOPHILUS; ORGANISM_TAXID  300852; STRAIN  HB8; GENE  TTHA1623; EXPRESSION_SYSTEM  ESCHERICHIA COLI; EXPRESSION_SYSTEM_TAXID  562; EXPRESSION_SYSTEM_VECTOR_TYPE  PLASMID; EXPRESSION_SYSTEM_PLASMID  PET11A: 2.20: 0.19
MRVFPVTLGPLQENAYLVETG-EGPVLIDPGDEPEKLLALFQTTGLIPLAILLTHAHFDHVGAVAPLVEALDLPV
YLHP-----L-DLPLYEGADLAARAWGLAIPKPPLPVRPLEEGMRLFGFQVLHLPGHSPGHVAFYDPEGAQVFSG
DLLFRGSVGRYDLPGADPKALFASLK-RLLSLPPETRVHPGHGPGTTLGLEARTNPFL..*

>P1;Metallo-beta-lactamase
sequence:Metallo-beta-lactamase:     :212 :     : ::: 0.00: 0.00
M-FKQIPLGPIQTNAYVLYNDDKEAVIFDPGGDAEALITWLKREQLTPLAILLTHAHFDHIGAVDAVRDTFSIPV
YLHTKERHWLEDPALNGSSRLTGRPITTAKPADHLLTNEKSLTIGTFTFSVFHTPGHSPGSVSYYYQKEAVLFSG
DVLFQQSIGRTDLRGGDHTLLLASIHNKILPLPERTIVASGHGPLTTIGQEMDHNPFLTGY--..*

Your suggestion is much more appreciated... Thank you
--
Zhao Yang