26 Apr
2019
26 Apr
'19
3:41 p.m.
On 4/26/19 3:40 AM, Andrea Graziadei wrote: > I am continuing to working with IMP/PMI for integrative modeling of a > protein complex using EM and crosslinking information. Ultimately, as in > the tutorial, I have 4 sets of restraints: excluded volume, > connectivity, crosslinks and gaussian. > > I would like to explore what weights to set.
Generally it shouldn't be necessary to modify the weights, except for possibly the EM restraint. There thus isn't really a protocol for optimizing the weights. Ideally each restraint would be Bayesian in nature such that weights are entirely unnecessary, although this isn't yet the case for EM.
Ben
--
ben@salilab.org https://salilab.org/~ben/
"It is a capital mistake to theorize before one has data."
- Sir Arthur Conan Doyle