Failed to build 1e6v example with multifit
Dear All,
Was anyone able to build 1e6v example?
I was trying to model complex I am currently working on, however with no luck. To test the software I've tried to model example 1e6v, provided along with the source code. Multifit align step produced asmb.combinations file with "NO SOLUTIONS FOUND".
Shouldn't example produce results? I was following the same instructions as in the 3sfd tutorial, which works.
Looking forward to hearing from you.
Best regards, Mateusz Korycinski
On 11/24/15 4:33 AM, Mateusz Koryciński wrote: > Was anyone able to build 1e6v example? > > I was trying to model complex I am currently working on, however with no > luck. To test the software I've tried to model example 1e6v, provided > along with the source code. Multifit align step produced > asmb.combinations file with "NO SOLUTIONS FOUND".
MultiFit is rather sensitive to the set of input parameters you feed it. But Keren, the author of MultiFit, is working to remedy this and may be able to help you.
Ben
Hi Mateusz,
If you open the map and your structures in their initial positions in Chimera, are they close in space to the map? For Multifit, the initial structures must be close to the box of the map. Maybe this is the reason.
See my previous response for explanations: https://integrativemodeling.org/archives/imp-users/msg00304.html
Best, Jan
On 30 Nov 2015, at 20:15, Ben Webb ben@salilab.org wrote:
> On 11/24/15 4:33 AM, Mateusz Koryciński wrote: >> Was anyone able to build 1e6v example? >> >> I was trying to model complex I am currently working on, however with no >> luck. To test the software I've tried to model example 1e6v, provided >> along with the source code. Multifit align step produced >> asmb.combinations file with "NO SOLUTIONS FOUND". > > MultiFit is rather sensitive to the set of input parameters you feed it. But Keren, the author of MultiFit, is working to remedy this and may be able to help you. > > Ben > -- > ben@salilab.org https://salilab.org/~ben/ > "It is a capital mistake to theorize before one has data." > - Sir Arthur Conan Doyle > _______________________________________________ > IMP-users mailing list > IMP-users@salilab.org > https://salilab.org/mailman/listinfo/imp-users
Hi Jan,
Thank you for your reply.
Yes, they are close in space to the map, in fact they are all packed in its center.
Best regards, Mateusz
On Mon, Nov 30, 2015 at 8:29 PM, Jan Kosinski kosinski@embl.de wrote:
> Hi Mateusz, > > If you open the map and your structures in their initial positions in > Chimera, are they close in space to the map? For Multifit, the initial > structures must be close to the box of the map. Maybe this is the reason. > > See my previous response for explanations: > https://integrativemodeling.org/archives/imp-users/msg00304.html > > Best, > Jan > > On 30 Nov 2015, at 20:15, Ben Webb ben@salilab.org wrote: > > On 11/24/15 4:33 AM, Mateusz Koryciński wrote: > > Was anyone able to build 1e6v example? > > I was trying to model complex I am currently working on, however with no > luck. To test the software I've tried to model example 1e6v, provided > along with the source code. Multifit align step produced > asmb.combinations file with "NO SOLUTIONS FOUND". > > > MultiFit is rather sensitive to the set of input parameters you feed it. > But Keren, the author of MultiFit, is working to remedy this and may be > able to help you. > > Ben > -- > ben@salilab.org https://salilab.org/~ben/ > "It is a capital mistake to theorize before one has data." > - Sir Arthur Conan Doyle > _______________________________________________ > IMP-users mailing list > IMP-users@salilab.org > https://salilab.org/mailman/listinfo/imp-users > > > > _______________________________________________ > IMP-users mailing list > IMP-users@salilab.org > https://salilab.org/mailman/listinfo/imp-users > >
participants (3)
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Ben Webb -
Jan Kosinski -
Mateusz Koryciński