Thanks Ben, It worked.
On Fri, Dec 4, 2020 at 11:55 AM Modeller Caretaker < modeller-care@salilab.org> wrote:
> On 12/4/20 9:08 AM, shivu shiv wrote: > > I am working on modeling a protein with two chains (heterodimer). > > I have used two separate pdb files for each chain and oriented the two > > template structures appropriately before the modeling. > > Modeller is able to model both the chains, however not preserving the > > orientation of both the chains. > > If you are confident of this orientation, put the two chains in a single > PDB file, as is done in the example in the manual: > https://salilab.org/modeller/9.25/manual/node29.html > > Modeller extracts internal distance restraints from each template > separately, so if your two chains are two separate templates, there will > be no inter-chain restraints. > > Ben Webb, Modeller Caretaker > -- > modeller-care@salilab.org https://salilab.org/modeller/ > Modeller mail list: https://salilab.org/mailman/listinfo/modeller_usage >