On 11/3/23 1:45 AM, rpw641--- via modeller_usage wrote: > As the Subject said, I want to make a heterotetramer model, and make > one of the chains by using two template. I know how to make > heterotetramer model or build a single chain with multitemplate. The > problem is that I don't know how to combine these two in my case.

This wouldn't be substantially different from the single chain case - you would just need to align each template with the right part of the model sequence. If you have a 4-chain template and you want to use a second template for part of one chain, your alignment would look something like

temp1 AAAA/BBBB/CCCC/DDDD* temp2 ----/----/CCCC/----* model AAAA/BBBB/CCCC/DDDD*

Ben Webb, Modeller Caretaker