Dear colleagues,

I have a problem in multichain modeller. according the template file in automodel folder, I created the input file and script file. however when I　run this script, I recieved warnings listed below. Who knows how to reslove the problem? Thanks a lot!

You can find many useful example scripts in the examples\automodel directory. It is recommended that you use Python to run Modeller scripts. However, if you don't have Python installed, you can type 'mod9.12' to run them instead.

d:\Program Files (x86)\Modeller9.12> d:\Program Files (x86)\Modeller9.12>cd examples

d:\Program Files (x86)\Modeller9.12\examples>cd automodel

d:\Program Files (x86)\Modeller9.12\examples\automodel>python model-multichain-d ec1.py > model-multichain-dec1.log Traceback (most recent call last): File "model-multichain-dec1.py", line 37, in <module> a.make() # do homology modeling File "d:\Program Files (x86)\Modeller9.12\modlib\modeller\automodel\automodel. py", line 96, in make self.homcsr(exit_stage) File "d:\Program Files (x86)\Modeller9.12\modlib\modeller\automodel\automodel. py", line 410, in homcsr aln = self.read_alignment() File "d:\Program Files (x86)\Modeller9.12\modlib\modeller\automodel\automodel. py", line 400, in read_alignment aln.append(file=self.alnfile, align_codes=self.knowns+[self.sequence]) File "d:\Program Files (x86)\Modeller9.12\modlib\modeller\alignment.py", line 78, in append allow_alternates) _modeller.ModellerError: read_al_373E> Protein specified in ALIGN_CODES(i) was n ot found in the alignment file; ALIGN_CODES( 1) = 4h10

d:\Program Files (x86)\Modeller9.12\examples\automodel>