Hi I try to model a chimeric protein based on FAQ 4 To test the method I took a PDB file and cut it in two parts and then try to model the complete molecule again based on an FAQ 4 I get the error: fit2xyz_E> number of equivalent positions < 3: 0 recover__> ERROR_STATUS >= STOP_ON_ERROR: 1 1 As I understand it there are not enough overlapping residues in the alignment. But that is exactly the point, I try to model a chimeric protein based on the independent fragments exactly as in FAQ 4. What is wrong? Does the templates have to be overlapping, and is FAQ 4 wrong? All files are attached Thanks in advance Kim ------------------------------------------------------------------------ Kim Vilbour Andersen, PhD Senior Scientist Maxygen Agern Allé 1 DK-2970 Hørsholm Denmark <<model.top.gz>> <<align.pir.gz>> <<1GR3_A2.pdb.gz>> <<1GR3_A1.pdb.gz>> Tel: +45 7020 5550 Direct: +45 4517 8473 Mobile: +45 2637 3727 Fax: +45 7020 5530 E-mail: http://www.maxygen.dk/ ------------------------------------------------------------------------
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model.top.gz
Description: model.top.gz
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align.pir.gz
Description: align.pir.gz
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1GR3_A2.pdb.gz
Description: 1GR3_A2.pdb.gz
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1GR3_A1.pdb.gz
Description: 1GR3_A1.pdb.gz