[modeller_usage] Advantages of manual protein sequence alignment between target and template [Very urgent please]

[rajesh rengaraj <>]

Hello protein builders;

   I am new to the Homology modeling field, I have
membrane protein as a target and I need to model this
protein, because of less sequence identity I suppose
to model this protein by manual protein sequence
alignment between the target and template. Let me know
the advantages of manual protein sequence alignment
and by this whether it is possible to improve the
structure of the target protein. I need some ideas
regarding this issue.


    Any suggestions would be appreciated.


  With regards,
   Rajesh Rangaraj.


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