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Re: [modeller_usage] Modeling a Chimera



Toty Santos wrote:
I have a bifuncional enzime with two domains. But in the PDB i have as a model different structures : One for domain A and other to domain B.

And i´d like to build a model with this two domains together. It is not a dimer, it a single enzime with two diferente domains. And i´d like to know tto, what i will do with the linker resudues between this both domains !!

See http://salilab.org/modeller/FAQ.html#1

In your model sequence, you may need to use the '/' character to denote a chain break between the two domains.

To ensure the correct orientation of the two domains, you will need to add additional information (e.g. a template sequence that partially overlaps the two, or manual distance restraints from experiments or other data).

I anybody can help me, please be detalist cause i am a start user
i am using Modeller 8v0

You should use Modeller 8v2 instead, which fixes some bugs in 8v0.

	Ben Webb, Modeller Caretaker
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             http://www.salilab.org/modeller/
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