Re: [modeller_usage] Modeling a Chimera

[Modeller Caretaker <modeller-care@ucsf.edu>]

Toty Santos wrote:
> I have a bifuncional enzime with two domains. But in the PDB i have as a 
> model different structures : One for domain A and other to domain B.
>
> And i´d  like to build a model with this two domains together. It is not a 
> dimer, it a single enzime with two diferente domains. And i´d like to know 
> tto, what i will do with the linker resudues between this both domains !!

See http://salilab.org/modeller/FAQ.html#1

In your model sequence, you may need to use the '/' character to denote 
a chain break between the two domains.

To ensure the correct orientation of the two domains, you will need to 
add additional information (e.g. a template sequence that partially 
overlaps the two, or manual distance restraints from experiments or 
other data).

> I anybody can help me, please be detalist cause i am a start user
> i am using Modeller 8v0

You should use Modeller 8v2 instead, which fixes some bugs in 8v0.

	Ben Webb, Modeller Caretaker
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