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[modeller_usage] BLK usage => mainy chains



Hello,

I try to build a modele with 2 protein templates. I want to modelise just one loop of 1pkg, part of it on the template 3A2M, the rest of the loop de novo. The rest of 1pkg must not be changed, so I put it as BLK:

P1;1pkg
structureX:1pkg:1:A:280:A:::: ..............................................................................
........................................--------------------------------------
-------------------------------------------...................................
..............................................................................
.................................................*
P1;3A2M
structureX:3A2M:283:A:305:A:::: ------------------------------------------------------------------------------
----------------------------------------QEDHADAIGRSVAGWIAGIEAVR---------------
------------------------------------------------------------------------------
------------------------------------------------------------------------------
-------------------------------------------------*
P1;KID
sequence:KID:1:A:361:A:::: ..............................................................................
........................................KRDSFICSKQEDHAEAALYKNLLHSKESSCSDSTNEYM
DMKPGVSYVVPTKADKRRSVRIGSYIERDVTPAIMEDDELALD...................................
..............................................................................
.................................................*


I get a model with 3 chains instead of one. How can I get a one-chain model?
All atoms in the pdb files are like "ATOM". Should I change it in "HETATM"?

Thanks a lot in advance,
Isaure

--
Isaure  Chauvot_de_Beauchêne


PhD Student
Laboratoire de Biolgie et Pharmacologie Appliquée (Dir J.-F. Mouscadet)
CNRS - Ecole Normale Supérieure de Cachan
Equipe BioInformatique, Modélisation et Dynamique Moléculaire (BIMoDyM) (Dir
Luba.Tchertanov)

61 Av du Président Wilson
94230 Cachan
France
--- Begin Message ---
Hello,

I try to build a modele with 2 protein templates. I want to modelise just a loop of 1pkg, part of it on the template 3A2M, the rest of the loop de novo. The rest of 1pkg must not be changed, so I put it as BLK:

P1;1pkg
structureX:1pkg:1:A:280:A::::
..............................................................................
........................................--------------------------------------
-------------------------------------------...................................
..............................................................................
.................................................*

P1;3A2M
structureX:3A2M:283:A:305:A::::
------------------------------------------------------------------------------
----------------------------------------QEDHADAIGRSVAGWIAGIEAVR---------------
------------------------------------------------------------------------------
------------------------------------------------------------------------------
-------------------------------------------------*

P1;KID
sequence:KID:1:A:361:A::::
..............................................................................
........................................KRDSFICSKQEDHAEAALYKNLLHSKESSCSDSTNEYM
DMKPGVSYVVPTKADKRRSVRIGSYIERDVTPAIMEDDELALD...................................
..............................................................................
.................................................*


I get a model with 3 chain instead of one. How can I get a one-chain model?
All atoms in the pdb files are like "ATOM". Should I change it in "HETATM"?

Thanks a lot in advance,
Isaure

--
Isaure  Chauvot_de_Beauchêne


PhD Student
Laboratoire de Biolgie et Pharmacologie Appliquée (Dir J.-F. Mouscadet)
CNRS - Ecole Normale Supérieure de Cachan
Equipe BioInformatique, Modélisation et Dynamique Moléculaire (BIMoDyM) (Dir
Luba.Tchertanov)

61 Av du Président Wilson
94230 Cachan
France

--- End Message ---