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Re: [modeller_usage] Modelling of multi-domain protein from oligomeric template

On 07/06/2011 07:40 AM, Chugunov Anton wrote:

What I expect, is a single-chain model that consists of four domains,
each built from respective template's chain according to the alignment.
What I get (as far as I see from the *.ini file, since no models output
due to high energy-function) is the first domain followed by a huge
non-structured loop
If the alignment you posted is the one you're actually using, then you have aligned DOM 2 through 4 with gaps in your template, so obviously Modeller has no structure to work with in that region. Remove all of those gaps from your template so that DOM-2 is aligned with BBBB, DOM-3 with CCCC and DOM-4 with DDDD. 

	Ben Webb, Modeller Caretaker
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