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[modeller_usage] minimize after filling the gaps




Hi,
On building the missing residues in a protein(under the option no change to rest of the protein), could i retain the cofactors.
Should i minimize the protein as a whole or build region specifically before performing any docking and simulation. I understand the building process itself perform some minimization. Isn't that enough?

Thanking You,



--
Mary Varughese
Research Scholar
School of Pure and Applied Physics
Mahatma Gandhi University
India