On 2/28/21 1:00 PM, Pedro Guillem wrote:
> I'm trying to model from a known template (ident 37%).
> The template is missing the first 3 residues in the structure.
>
> - I start reading the template at position 4.
> - I add 3 dashes to the beginning of the template to compensate for the
> missing residues. (otherwise I get a "residue mismatch" error from modeller)
Dashes/gaps do not have any effect on which residues are read from the
PDB file; this is controlled entirely by the PIR header (the line
starting with "structureX", where you - apparently correctly - have 4 as
the start residue number). Dashes/gaps only control the correspondence
between target and template residues.
> Here is a bit of my ali file. I want the TRL of the template to align
> with the TRM of the sequence.
Your alignment looks fine to me, assuming by "..." you mean "the rest of
the sequence goes here". (A literal "." has a special meaning to
Modeller; read in the manual about modeling with ligands.)
> When it finishes, the position of TRM has a different offset in relation
> to TRL