-------- Original Message --------
Subject: modeling crosslinked homodimeric protein
Date: Mon, 26 Mar 2007 10:29:24 +0530
From: Archana Chavan <>
To:
hello everybody,
i am tryong to model the structure of enzyme which is crosslinked
homodimeric protein
two identical chains of protein are crosslinked in opposite orientation
to form the two compete active sites.
that is, both chains contribute fo the foramtion of aech of the active
site. (there are 64 hydrogen bonds involved in inetaction between two
chains)
i tried to model to chains seperatly and superimpossed bsed on template,
but the orientation of the chains is not giving the proper active site, so
how i can model the whole protein, i.e. both the chains simultaneously
so that i can get full active site?
or is there any way by which seperatly modeled chaines can be put together?
please suggest any solution for this problem.
Thanks in advance.
--
Archana Chavan
MS (Pharm)Pharmacoinformatics
National Institute of Pharmaceutical Education and Research (NIPER)
Mohali-160 062
Punjab, India.
phone:+91-(0)-9888847223
Ben Webb, Modeller Caretaker
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