Good morning, I'm looking at trying to build models of a set of membrane-bound proteins via structural homology, and I was wondering if there are any special caveats that apply to this situation.
Andrew D. Fant wrote: > I'm looking at trying to build models of a set of membrane-bound > proteins via structural homology, and I was wondering if there are any > special caveats that apply to this situation.
Modeller will not treat such proteins specially, so whether it builds reasonable models depends on whether they _need_ to be treated specially. If you have reasonable sequence identity, it is likely to do a good job at building models, since by construction your model will look like the template where the two are aligned - but in low seq.id. cases it has to rely more on its library of known protein structure characteristics, and this is constructed using only globular proteins, so may not accurately represent any special cases.
Ben Webb, Modeller Caretaker