Dear Modellers

I have a manually constructed model protein, which I would like to refine using the MODELLER default schedule. I have attempted this using the protocol based on question 12 in the MODELLER FAQ. However, I find that MODELLER reads the model.ini file, and then extracts restraints from the templates, which are then used in the optimization of the model. I would like to refine my model based only upon my initial conformation. Is this possible do do, and if so, how should I go about it?

Thanks in advance,

Derek Smith.

************************************************************************* Derek Smith phone: (01904) 432550 Structural Biology Laboratory email: derek@yorvic.york.ac.uk Department of Chemistry fax: (01904) 410519 University of York, Heslington, York. YO10 5DD United Kingdom. *************************************************************************