•·.·´¯`·.·• Iƒƒ¥ •·.·´¯`·.·• wrote: > I have done alignment through salign as mentioned in advance tutorial > but i think it dosent gives good alignment.File AIRE_mult.ali and > align2d_mult are attached. > Thats why I separately aligned template sequences with protien sequence > using align2d.py one by one and then wrote the result in one file by > myself i-e. Mannual_Alignment.ali. > > Is this the rite way ? or M doing wrong? > > Which way should I use ?
There is no one "right way" to make an alignment. In your case you have huge regions of the sequence with no aligned template. Modeller is not going to be able to build an accurate model. So you would be better off only building models of the parts of the sequence for which you do have a template.
Ben Webb, Modeller Caretaker